Systems and Network Biology of MicroRNA

Brief Introduction on microRNA
  • MicroRNAs (miRNAs) are small (approximately 22 nt) noncoding RNAs that play an important role in the regulation of various biological processes through their interaction with cellular messenger RNAs.
  • They are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication.
  • Extracellular miRNAs in serum, plasma, saliva, urine and other body fluids have recently been shown to be associated with various pathological conditions including cancer.
  • miRNAs circulate in the bloodstream in a highly stable, extracellular form, thus they may be used as blood-based biomarkers for cancer and other diseases. Circulating miRNAs are protected by encapsulation in membrane-bound vesicles such as exosomes, but the majority of circulating miRNAs in human plasma and serum cofractionate with Argonaute2 (Ago2) protein, rather than with vesicles.

MicroRNA Analysis on High Throughput NGS Datasets

  • Qualifty control
  • Discard all the reads with a length < 15.
  • Discard quality under 20 (in phred score, a score of 20 represent 99% of base call acuracy ).
  • Filtering on other RNA species (snoRNA, tRNA, piRNA) via Blastn or ssearch.

More Relavent Info on microRNA

First set up on August 26, 2012, last updated on October 10, 2012 by Dr. Jeff Chen